2. de Kretser DM, Kerr JB. The cytology of the testis. In: Knobil E. Neill J, Greenwald GS. Markest CL (eds.) Raven Press: New York,1994: 1177-1290
3. Colgan T J, Bedard YC. Strawbridge HTG, Buckspan MB, Klotz PG. Reappraisal of the value of testicular biopsy in the investigation of infertility. Fertil Steril 1980: 33: 56-60
3. Martin-de-Pan RC, Campana A. Physiopathology of spermatogenic arrest. Fertil Steril 1993; 60: 937-946
4. Chabot B, Stephenson DA, Chapman VM et al. The proto-oncogene c-kit encoding a transmembrane tyrosine kinase receptor maps to the mouse W locus. Nature 1988; 335: 88-89
5. Anderson DM, Lyman SD, Baird A et al. Molecular cloning of mast cell growth factor, a hematopoietin that is active in both membrane bound and soluble forms. Cell 1990; 63: 235-243
6. Albanesi C, Geremia R, Giorgio M et al. A cell and developmental stage-specific promoter drives the expression of a truncated c-kit protein during mouse spermatid elongation. Development 1996; 122: 1291-1302
7. Franchimont P, Millet D, Vendrely E. Letawe J, Legros JJ, Netter A. Relationship between spermatogenesis and serum gonadotrophin levels in azoospermia and oligospermia. J Clin Endocrinol Metab 1972; 34: 1003
8. Glezerman M. Etiology of fertility disturbances in man. In: Disturbances in male fertility. Springer-Verlag: Berlin. 1982
9. Dubin L, Amelar RD. Etiological factors in 1294 consecutive cases of male infertility. Fertil Steril 1971: 22: 469
10. Chandley AC, Edmond P, Maclean N, Fletcher J, Watson ES. Cytogenetics and infertility in man. Results of a five year survey of men attending an infertility clinic. II. Testicular histology and meiosis. Ann Hum Genet 1976; 40: 156
11. Silber SJ, Rodriguez-Rigau LI Quantitative analysis of testicular biopsy: determination of partial obstruction and prediction of sperm count after surgery for obstruction. Fertil Steril 1981; 36: 480-485
12. Rajwanshi A, Indudhara R, Goswami AK, Radhika S, Das A, Sharma SK, Vaidyanathan S, Datta BN. Fine needle aspiration cytology in azoospermic males. Diagnostic cytopathology 1991; 7: 3-6
13. Edwards RG, Fishel SB et al. Factors influencing the success of in-vitro fertilisation for alleviating human infertility. J In Vitro Fert Embryo Transfer 1984; 1: 3-23
14. Fishel S, Green S, Bishop M, Thornton S, Hunter A, Fleming S, Al-Hassan S. Pregnancy after intracytoplasmic injection of spermatid. Lancet 1995; 345: 1641-1642
15. Palermo G, Joris H, Devroey P, Van Steirteghem AC. Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte. Lancet 1992; 340: 17-18
16. Van Steirteghem AC, Nagy Z, Joris H et al. High fertilisation and implantation rates after intracytoplasmic sperm injection. Hum Reprod 1993; 8: 1061-1066
17. Silber SJ. What forms of male infertility are there left to cure. Hum Reprod 1995; 10: 503-504
18. Silber S J. Results of microsurgical vasoepididymostomy: role of epididymis in sperm maturation. Hum Reprod 1989; 4: 298-303
19. Silber SJ, Nagy ZP, Liu J, Godoy H, Devroey P, Van Steirteghem AC. Conventional in vitro fertilisation versus intracytoplasmic sperm injection for patients requiring microsurgical sperm aspiration. Hum Reprod 1994: 9: 1705-1709
20. Devroey P, Liu J, Nagy Z, Tournaye H, Silber SJ, Van Steirteghem AC. Normal fertilisation of human oocytes after testicular sperm extraction and intracytoplasmic sperm injection. Fertil Steril 1994; 62: 639-641
21. Silber SJ, Van Steirteghem AC, Liu J, Nagy Z, Tournaye H, Devroey P. High fertilisation and pregnancy rates after intracytoplasmic sperm injection with sperm obtained from testicle biopsy. Hum Reprod 1995; 10: 148-152
22. Schulze C. Sertoli cells and leydig cells in man. In: Advances in anatomy, embryology and cell biology, vol 88. Springer-V erlag: Berlin, Heidelberg, New York, 1984.
23. Austin CR. The mammalian egg. Blackwell scientific publications; Oxford, 1961
24. Edwards RG, Tarin JJ, Dean N, Hirsch A, Tan SL. Are spermatid injections into human oocytes now mandatory? Hum Reprod 1994; 9: 2217-2219
25. Ogura A, Yanagimachi R. Round spermatid nuclei injected into hamster oocytes form pronuclei and participate in syngamy. Biol Reprod 1993; 48: 219-225
26. Ogura A, Yanagimachi R, Usui N. Behaviour of hamster and mouse round spermatid nuclei incorporated into mature oocytes by electrofusion. Zygote 1993; 1: 1-8
27. Ogura A, Matsuda J, Yanagimachi R. Birth of normal young after electrofusion of mouse oocytes with round spermatid. Proc Natl Acad Sci 1994; 91: 7460-7462
28. Sofikitis N, Zavos P, Koutselinis A, Mourtzinis D, Loutradis D, Glanzounis G. Achievement of pregnancy after injection of round spermatid (RS) nuclei into rabbit oocytes and embryo transfer: a possible mode of treatment for men with spermatogenic arrest at the spermatid stage. J Urol 1994; 5(suppl): 151
29. Vanderzwalmen P, Lejeune B, Nijs M, Bertin GS, Vandamme B, Schoysman R. Fertilisation of an oocyte microinseminated with a spermatid in an in vitro fertilisation programme. Hum Reprod 1995; 10:502-503
30. Tesarik J, Mendoza C, Testart J. Viable embryos from injection of round spermatids into oocytes. The New England Journal Of Medicine 1995; 333: 525
31. Aslam I, Fishel S. The use of spermatids for human conception. In: Hansson V, Levy FO, Tasken K (eds.) Signal transduction in testicular cells. Springer-Verlag: Berlin, Heidelberg, New York, 1996; 271-286
32. Fishel S, Aslam I, Tesarik J. Spermatid conception: a stage too early, or a time too soon? Hum Reprod 1996; 11: 1371-1375
33. Perreault SD. Regulation of sperm nuclear reactivation during fertilisation. In: Bevister BD, Cummins J, Roldam ERS (eds.) Fertilisation in mammals. Serono symposia: Norwell 1990; 285-296
34. Itoh T, Ausio J, Katagiri C. Histone H l variant as sperm-specific nuclear protein of Rana catesbeiana, and their role in maintaining a unique condensed state of sperm chromatin. Mol Reprod Dev 1997; 47: 181-190
35. Meistrich ML. Histone and basic nuclear protein transition in mammalian spermatogenesis. In: Hnilica CS, Stein GS, Stein JL (eds.) Histones and other basic nuclear proteins. CRC press: Boca Raton, FL, USA, 1989; 165-182
36. Choudhary SK, Wykes SM, Mohamed AK et al. A haploid expressed gene cluster exists as a single chromatin domain in human sperm. J Biol Chem 1995; 270: 8755-8762
37. Barton SC, Surani MAH, Norris ML. Role of paternal and maternal genomes in mouse development. Naturę 1984; 311: 374-376
38. McGrath J, Solter D. Completion of mouse embryogenesis requires both the maternal and paternal genomes. Cell 1984; 37:179-183
39. Surani MZH. Evidences and consequences of differences between maternal and paternal genomes during embryogenesis in the mouse. In: eds? Experimental approaches to mammalian embryonic development. Cambridge University press: Cambridge.1986: 401-435
40. Cedar H. DNA methylation and gene expression. In: Razin A, Cedar H. Riggs AD (eds.) DNA methylation: biochemistry and biological significance. Springer-Verlag: New York, 1984; 147-164
41. Bartolomei MS. Zemel S, Tilghman SM. Parental imprinting of the mouse H19 gene. Nature 1991; 351: 153-155
42. Rainier S, Johnson LA, Dobry C J, Ping A, Grundy PA,
Feinberg AP. Relaxation of imprinted genes in human cancer. Naturę 1993; 362: 747-749
43. Aslam I, Scobie G, Fishel S. Genomic imprinting in the spermatogenic cells used for in-vitro fertilisation. Journal of Reproduction and Fertility 1996; Abstract series no. 18: l
44. Kimura Y, Yanagimachi R. Mouse oocytes injected with testicular spermatozoa or round spermatids can develop into normal offspring. Development 1995(a); 121: 2397-2405
45. Kimura Y, Yanagimachi R. Development of normal mice from oocytes injected with secondary spermatocyte nuclei. Biology of Reproduction 1995(b); 53: 855-862
46. Ma K, Inglis JD, Sharkey A, Bickmore WA, Hill RE, Prosser EJ, Speed RM, Thomson EJ, Jobling M, Taylor K, Wolfe J, Cooke HJ, Hargreave TB, Chandley AC. A Y chromosome gene family with RNA-binding protein homology: candidates for azoospermia factor AZF controlling human spermatogenesis. Cell 1993; 75: 1287-1295
47. Reijo R. Lee TY, Salo P, Alagappan R et al. Diverse spermatogenic defects in humans caused by Y chromosome deletions encompassing a novel RNA-binding protein gene. Naturę Genet 1995; 10: 383-392
48. Qureshi S J. Ross AR. Ma K et al. Polymerase chain reaction screening for Y chromosome microdeletions: a first step towards the diagnosis of genetically determined spermatogenic failure in men. Mol Hum Reprod 1996; 2: 775-779
49. In't Veld PA, Halley DJJ, Hemel JOV Niermeijer MF, Dohle G, Weber RFA. Genetic counselling before intracytoplasmic sperm injection. Lancet 1997; 350: 490
50. Vogt PH, Edelmann A, Kirsch S et al. Human Y chromosome azoospermia factors (AZF) mapped to different subregions in Yql 1. Hum Molec Genet 1996; 5: 933-943
51. Mulhall JP, Reijo R, Alagappan R, Brown L. Page D, Carson R, Oates RD. Azoospermic men with deletion of the DAZ gene cluster are capable of completing spermatogenesis: fertilisation, normal embryonic development and pregnancy occur when retrieved testicular spermatozoa are used for intracytoplasmic sperm injection. Hum Reprod 1997; 12: 503-508
52. Boveri T. Zellen-studien: uber die natur der centrosomen IV. Jena: Germany, 1901
53. Schatten G, Simerly C, Schatten H. Maternal inheritance of centrosomes in mammals? Studies on parthenogenesis and polyspermy in mice. Proc Natl Acad Sci USA 1991; 88: 6785-6789
54. Simerly C, Wu G, Zoran S et al. The paternal inheritance of the centrosome, the cell's microtubule-organising centre, in humans and the implications for infertility. Nature medicine 1995: 1:47-52
55. Palermo G, Munne S, Cohen J. The human zygote inherits its mitotic potential from the male gamete. Hum Reprod 1994; 9: 1220-1225
56. Vitullo AD. Ozil JR Repetitive calcium stimuli derive meiotic resumption and pronuclei development during mouse oocyte activation. Developmental biology 1992; 151: 128-136
57. Homa ST. Carroll J, Swann K. The role of calcium in mammalian oocyte maturation and egg activation. Hum Reprod 1993: 8:l274-1281
58. Tesarik J. Sousa M. Testart J. Human oocyte activation after intracytoplasmic sperm injection. Hum Reprod 1994; 9: 511-518
59. Swann K. A cytosolic sperm factor stimulates repetitive calcium increases and mimics fertilisation in hamster eggs. Development 1990: 110: 1295-1302
60. Dozortsev D. Rybouchkin A, DeSutter P, Qian C, Dhont M. Hurnan oocyte activation following intracytoplasmic injection: the role of the sperm cell. Hum Reprod 1995; 10: 403-407
61. Sousa M. Tesarik J. Ultrastructural analysis of fertilisation failure after intracytoplasmic sperm injection. Hum Reprod 1994; 9: 2374-2380
62. Bellve R A. Purification, culture and fractionation of spermatogenic cells. Methods in enzymology 1993; 225: 84-113
63. Shepherd WR, Millette FC, DeWolf GW Enrichment of primary pachytene spermatocytes from the human testes. Gametę research 1981; 4: 487-498
64. Narayan P, Scott BK, Millette CF, DeWolf WC. Human spermatogenic cell marker proteins detected by two- dimensional electrophoresis. Gamete Research 1983; 7: 227-239
65. Aslam I Robins R A, Dowell K, Fishel S. Isolation, purification and assessment of viability of spermatogenic cells from testicular biopsies of azoospermic men. Hum Reprod 1998;in press.
66. Aslam I, Fishel S. Short-term in-vitro culture and cryopreservation of spermatogenic cells used for human in- vitro conception. Hum Reprod 1997; in press.
67. Fishel S, Green S, Hunter A, Lisi F, Rinaldi L, Lisi R, McDermott H. Human fertilisation with round and elongated spermatids. Hum Reprod 1998; 12: 336-340
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